| Volume 27, Issue 1 | |
| Photobiomodulation Effect of 850 nm Near-infrared Diode Laser on the Release of M1-related Cytokines Inmonocytic THP-1 Cells | 5-14 |
| Ruwaidah A. Mussttaf, Mayyadah Hasan Rhaif Al-Sahlanee, Numan S. Dawood, David F. L. Jenkins, Awadhesh N. Jha | |
| doi: 10.7546/ijba.2023.27.1.000857 | |
| [ +/- how to cite ][ +/- abstract ][ full text ] | |
| Ruwaidah A. Mussttaf, Mayyadah Hasan Rhaif Al-Sahlanee, Numan S. Dawood, David F. L. Jenkins, Awadhesh N. Jha (2023) Photobiomodulation Effect of 850 nm Near-infrared Diode Laser on the Release of M1-related Cytokines Inmonocytic THP-1 Cells, Int J Bioautomation, 27 (1), 5-14, doi: 10.7546/ijba.2023.27.1.000857 | |
| Abstract: Photobiomodulation (PBM) is a form of the use of visible red and Near-infrared (NIR) light at low power, where a laser light photon is absorbed at the electronic level, without heat production. PBM can be applied in wide range of treatment to help the wound, inflammation, edema, and pain reduction. However, there is a lack of scientific documentation regarding its actual effects. Objectives: This study assesses the impact of PBM on the release of M1-related cytokine in monocyte cells with particular emphasis on interleukin-1β (IL-1β) and Tumour Necrosis Factor α (TNF-α). Methods: Tamm-Horsfall Protein 1 (THP-1) macrophages M1 cells have been exposed to the light from the diode laser of 850 nm at different doses (0, 0.6, 1.2 and 3.6 J/cm2). The release of cytokines was determined by enzyme-linked immunosorbent assay, after different periods of incubation (0, 12, 24, and 48 hours) post-irradiation. The proliferation of fibroblast cells suspended in irradiated M1-supernatent was evaluated for the same periods of incubation. Results: The results showed that PBM significantly enhanced M1-related cytokine release (p < 0.05). Obviously, IL-1β increased post-irradiation at 1.2 J/cm2 more than other doses for all incubation periods. TNF-α was decreased significantly after two days of irradiation (p < 0.005) for all doses. A significant increase in fibroblast proliferation (p < 0.005) was observed concomitant with the boost of cytokine release. Conclusion: This in vitro study has demonstrated that the PBM of the 850 nm diode laser therapy can enhance M1-related cytokine release, which in turn increases the proliferation of fibroblast cells. Moreover, PBM at 850 nm plays an anti-inflammatory role, which manifested by decreasing the level of TNF-α. Therefore, this therapy may be able to accelerate the wound healing process.
Keywords: Photobiomodulation, Cytokines, Anti-inflammatory effect, Macrophages | |
| Comparison of Calcified Plaque Volume in Coronary Arteries using Filtered Back Projection and Intelli IP Advanced at CT-scan 128 Slice | 15-24 |
| Naufal Arya Pratama, Lailatul Muqmiroh, Amilia Kartika Sari, Widiana Ferriastuti, Falentina Syivasari, Muhaimin, Sidarta Prassetyo, Cong Tien Nguyen, Phan Anh Phuong, Vu Dang Luu | |
| doi: 10.7546/ijba.2023.27.1.000878 | |
| [ +/- how to cite ][ +/- abstract ][ full text ] | |
| Naufal Arya Pratama, Lailatul Muqmiroh, Amilia Kartika Sari, Widiana Ferriastuti, Falentina Syivasari, Muhaimin, Sidarta Prassetyo, Cong Tien Nguyen, Phan Anh Phuong, Vu Dang Luu (2023) Comparison of Calcified Plaque Volume in Coronary Arteries using Filtered Back Projection and Intelli IP Advanced at CT-scan 128 Slice, Int J Bioautomation, 27 (1), 15-24, doi: 10.7546/ijba.2023.27.1.000878 | |
| Abstract: Calcified plaque has a major effect on increasing plaque volume which can increase the risk of Coronary Artery Disease (CAD). The use of Filtered Back Projection (FBP) on CT-scans still has flaws in terms of image quality that can result in errors in the calculation of plaque volume. Intelli IP Advanced is hitachi CT-scan’s Iterative Reconstruction (IR) type and can improve image quality compared to FBP. The purpose of this study is to find out the comparison of calcified plaque volume in coronary arteries using FBP and Intelli IP Advanced. This study used 38 samples of coronary artery blood vessels that had calcified plaque and reconstructed using the reconstruction of FBP and Intelli IP Advanced algorithms (Levels 1-3). Plaque calculation is done automatically using aquarius intuition edition application on CT-scan Hitachi SCENARIA 128 Slice. The results in this study found significant differences (p < 0.05) in calcified plaque volume results with the use of FBP and Intelli IP Advanced algorithm reconstruction (Levels 1-3). However, significant differences are found only in algorithm reconstruction pairs (Intelli IP Advanced Level 1 and Level 3) that have a p < 0.05. The conclusion is that IR affects the volume value of calcified plaque.
Keywords: Calcified plaque, Filtered back projection, Intelli IP Аdvanced | |
| Antibiotic Resistance of Heterotrophic Bacteria Isolated from Drinking Water – from the Water Source to the Consumers’ Taps | 25-38 |
| Zvezdimira Tsvetanova, Hristo Najdenski | |
| doi: 10.7546/ijba.2023.27.1.000882 | |
| [ +/- how to cite ][ +/- abstract ][ full text ] | |
| Zvezdimira Tsvetanova, Hristo Najdenski (2023) Antibiotic Resistance of Heterotrophic Bacteria Isolated from Drinking Water – from the Water Source to the Consumers’ Taps, Int J Bioautomation, 27 (1), 25-38, doi: 10.7546/ijba.2023.27.1.000882 | |
| Abstract: The aim of this study was to determine the antibiotic resistance (ABR) phenotype of heterotrophic bacteria isolated from a Bulgarian drinking water supply system. The disc diffusion method of Kirby-Bauer was used in the bacterial isolates susceptibility testing toward 13 antibiotics from 7 classes. The ABR phenotype of 233 bacterial strains was determined and the influence of drinking water treatment and its transportation in the water supply network on the ABR prevalence was assessed. Among the isolates, the ABR incidence was low or medium, only tetracycline resistance was very rare, and no gentamicin resistance was detected; 42% of the isolates were resistant to one class of antibiotics, and 11% exhibited multiple resistance. Water chlorination has led to restructuring of the aquatic community and changes of the predominant ABR phenotypes. The ABR phenotype of the isolates differed depending on the drinking water sampling point demonstrating the local influence of the water supply network. Although there is no regulation of the ABR of drinking water, the data demonstrated prevalence of bacterial resistance to different antibiotics and need of a greater concern the microbiological water quality.
Keywords: Antibiotic resistance, Heterotrophic bacteria, Drinking water, Drinking water distribution system | |
| Study on the Phototoxicity and Antitumor Activity of Plant Extracts from Tanacetum vulgare L., Epilobium parviflorum Schreb., and Geranium sanguineum L. | 39-50 |
| Inna Sulikovska, Elena Ivanova, Ivaylo Ivanov, Donka Tasheva, Mashenka Dimitrova, Biliana Nikolova, Ivan Iliev | |
| doi: 10.7546/ijba.2023.27.1.000888 | |
| [ +/- how to cite ][ +/- abstract ][ full text ] | |
| Inna Sulikovska, Elena Ivanova, Ivaylo Ivanov, Donka Tasheva, Mashenka Dimitrova, Biliana Nikolova, Ivan Iliev (2023) Study on the Phototoxicity and Antitumor Activity of Plant Extracts from Tanacetum vulgare L., Epilobium parviflorum Schreb., and Geranium sanguineum L., Int J Bioautomation, 27 (1), 39-50, doi: 10.7546/ijba.2023.27.1.000888 | |
| Abstract: Our country is rich in medicinal plants with a thousand-year tradition of use for therapeutic and cosmetic purposes. World Health Organization estimates that around 70% of population is using traditional (folk) medicine to cure various ailments. In many cases, herbal preparations prove to be useful, but some of them can also exert toxic effects. Therefore, herbal extracts should be tested both as curatives and for safety application. The aim of the present work was to investigate extracts from Tanacetum vulgare (TVA), Epilobium parviflorum (EPE) and Geranium sanguineum (GSA), obtained by different organic solvents, for their phototoxicity and anticancer activity. The standard BALB/c 3T3 Neutral Red Uptake phototoxicity/cytotoxicity assay was used to evaluate the possible phototoxic properties of the extracts. The antitumor activity of the extracts was studied in vitro on a panel of human tumor cell lines in comparison to the non-tumorigenic MCF-10A cells. The selectivity indices and the photo-irritancy factors were determined. The results show that the studied extracts are not phototoxic at concentration range from 4 to 1000 µg/ml. Determined photo-irritancy factors (PIF) for the extracts was < 2 (PIF for EPE = 1.72, GSA = 1.04, TVA = 1.43), which assigns them to the category of non-phototoxic substances. In addition, at concentrations lower than < 60 µg/ml, no statistically significant cytotoxicity was observed. The selectivity index was calculated relative to the IC50 value for non-tumorigenic MCF-10A cells. The highest selective index obtained with respect to the HT-29 cells was shown by all testing extracts (selective index (SI) for EPE = 2.6, GSA = 2.4 and TVA = 2.5), to the HeLa cells by extract GSA (SI = 2.0) and to the PC3 cells by extracts GSA (SI = 2.6) and TVA (SI = 2.0). Presented data shows that the studied extracts have a high level of photosafety with a promising antitumor activity in certain cell lines.
Keywords: Phototoxicity test, Plant extracts, Prolyl oligopeptidase | |
| Quantitative EEG Based on Direct Recording and Source Localization Estimation in Repetitive Hand Motor Activity | 51-68 |
| Masitoh, Suprijanto, Finda D. Putri, Vebi Nadhira | |
| doi: 10.7546/ijba.2023.27.1.000897 | |
| [ +/- how to cite ][ +/- abstract ][ full text ] | |
| Masitoh, Suprijanto, Finda D. Putri, Vebi Nadhira (2023) Quantitative EEG Based on Direct Recording and Source Localization Estimation in Repetitive Hand Motor Activity, Int J Bioautomation, 27 (1), 51-68, doi: 10.7546/ijba.2023.27.1.000897 | |
| Abstract: Electroencephalography (EEG) has been widely used in clinical practice as a tool to guide an efficient post-stroke functional recovery program and adjust the rehabilitation training program. EEG has some advantages in temporal resolution, portability, and relatively low cost. Motor-related brain activities can be quantified by measuring the mu rhythm’s event-related desynchronization (ERD), acquired from a direct surface EEG recording. The common problem of direct surface EEG is the crosstalk between brain regions that are unrelated to the sensorimotor cortex. This problem can be solved through the EEG source localization technique. Essentially, the idea of source reconstruction is to estimate the primary cortical current densities during the given EEG recording across the head surface. The recent work aims to compare EEG power spectral density (PSD) analysis and source localization. Modification of exact-Low Resolution Brain Electromagnetic Tomography (eLORETA) was used as an EEG source localization method. EEG data recording was performed on a healthy, able-bodied male subject using 21 electrodes based on the case study’s 10-20 electrode placement system. The subject was asked to perform basic hand movements, i.e., grasping with and without hand load. Mu power was calculated from the electrodes that are located around the motor cortex (C3 and C4), while the average current density was calculated from the Brodmann Area (BA) 4 and 6, both during grasping and relaxation (baseline). The comparison showed that during grasping, there was a decrease in aver-age current density around BA-4 and BA-6 and a decrease in mu power at C3 and C4 when compared to baseline. These results indicate: (1) the activity of mu rhythm’s ERD around the sensorimotor cortex during hand movement; and (2) the possibility of using the EEG source localization method as an alternative biofeedback modality.
Keywords: Brain imaging, Rehabilitation, Direct EEG recording, Source localization, Current density, Mu rhythm |
Sponsored by National Science Fund of Bulgaria, Grant No KP-06-NP4-25, 2023
© 2023, BAS, Institute of Biophysics and Biomedical Engineering