Biomacromolecules and Biomolecular Interactions
Head of Department
Prof. Stefka Germanova Taneva, DSc.
- E-mail: sgtaneva
gmail.com
- Phone: (359) 2 9792625
- Fax: (359) 2 8723787
- Address: “Acad. G. Bonchev” Str., Block 21, Room 314, Sofia 1113, Bulgaria
Staff
- Assoc. Prof. Sashka Boychova Krumova, PhD, sashkaobzor.bio21.bas.bg
- Assoc. Prof. Tonya Dimitrova Andreeva, PhD, t_andreevaabv.bg
- Assoc. Prof. Svetla Zhelyazkova Todinova, PhD , todinovaabv.bg
- Senior Assist. Prof. Roumiana Todorova Todorova, PhD, todorova
bio21.bas.bg
- Sen. Assist. Prof. Svetozar Dimitrov Stoichev, PhD, svetlio85abv.bg
- Assist. Prof. Avgustina Krasimirova Danailova, PhD, avgustina_danailovaabv.bg
- Assist. Prof. Nia Petrova, PhD student, petrova_niayahoo.com
- Nikoleta Nikolaeva Chenkina, specialist, nikoleta_chenkinaabv.bg
- Ina Nikolai Giosheva, specialist, ina_giabv.bg
- Ognian Konstantinov Apostolov, specialist, oapostolovgooglemail.com
Main research topics
See detailed information >
Dynamics of biological macromolecules and structure/function relationship. Thermal stability of proteins and complex biological systems. Macroorganization of pigment-protein complexes, factors modulating their conformation, integrity and function (lipids, biogenic organic compounds, steroids, ion strength and others). Application of thermodynamics in medicine – microcalorimetry of serum proteome (screening technology for diagnostics and monitoring of wide range of diseases).
Research projects
Current projects
- Encapsulation of peptide-based drugs in natural, synthetic and hybrid nano- and microcapsules, 2017-2019, Institute of Biophysics, University of Natural Resources and Life Sciences – BOKU, Vienna, DAAD Austria 01/7, 2017
- Coordinator: Assoc. Prof. Tonya Andreeva
- Human erythrocyte’s ageing in healthy and pathological subjects, studied by differential scanning calorimetry, atomic force microscopy and biochemical patterns
Italy, Institute for the Structure of Matter, Italian National Research Council, Rome, Italy, Bilateral cooperation – 2016-2018
Coordinator: Prof. Stefka Taneva
- Surface biofunctunalization of solid surfaces for bioelectronic applications,
Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Hungary, Bilateral cooperation – 2016-2018
Coordinator: Prof. Stefka Taneva
- 4. Factors modulating the structural organization, stability and oxidative stress resistance of cyanobacterial thylakoid membranes
Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Hungary, Bilateral cooperation – 2016-2018
Coordinator: Assoc. Prof. Sashka Krumova
- Investigation of the stability of DNA metabolic enzymes and thier interactions with ligands by means of spectroscopic, biochemical and thermodynamic methods
Bulgarian Science Fund, ДРИЛА-01-11, 2013-2015
Coordinator: Assoc. Prof. Sashka Krumova
- Role of photosystem II ordering in thylakoid membranes of higher plants for their resistance towards drought stress, ДФНП-17-138, 2017
Coordinator:Res. Assoc. Nia Petrova
- Calorimetric Plasma Proteome Assay for Early Diagnosis of Malignant Diseases. In vivo experimental models of colorectal carcinoma, ДФНП 17-133/2017
Coordinator:PhD student Avgustina Danailova
Past projects
- Regulation of the functions and organization of the photosynthetic apparatus by brassinosteroids
Bulgarian National Science Fund, 2009-2013
- A detailed study of the role of yeast linker histone Hho1p in the processes of chromatin remodeling
Joint project with Institute of Molecular Biology, BAS
Bulgarian National Science Fund, 2009-2012
- Light-energy conversion and molecular dynamics in retinal proteins. Bioelectronical application
Academy Exchange Project, with Prof. P. Ormos, Institute of Biophysics, Hungarian Academy of Sciences, 2010-2015
- Optimized formation of monomolecular layers from the major light harvesting complex of photosystem II on the phase boundary buffer/air
Institute of Biophysics and Biomedical Engineering, BAS, 2011-2012
- Thermodynamic investigation of blood serum, novel approach for monitoring of haematological (multiple myeloma) diseases
Institute of Biophysics and Biomedical Engineering, BAS, 2011-2013
- Role of supramolecular organization of PSII – LHCII complex in isoprene emitting plants and its relation to thermoprotection
Joint project of IBPhBME and IPBG, BAS, 2010-2012
- Carotenoid-lipid interactions in liposomes and antennae complexes
Joint project of Sofia University and IBPhBME, BAS
- Colloid aspects of nanoscience: nanostructures at interfaces in bulk and in three-phase contact zones
Bulgarian National Science Fund, Project module NT-01/03: „Modified liquid and solid surfaces with adjustable nanostructures and properties”, 2006-2010.
- Developing a biosensor using photoexcitable membranes
Indo-Bulgarian intergovernmental programme of cooperation in science and technolgy, F.M. University, Dept. of Biosciences and Biotechnology, with Prof. A. Misra, 2007-2009.
- Biofunctionalized surfaces at solid-liquid interfaces
Institute of Biophysics, Hungarian Academy of Sciences, Academy Exchange Project, with Prof. P.Ormos, 2006-2009.
- Molecular mechanism of biological light energy transduction
Institute of Biophysics, Hungarian Academy of Sciences, Academy Exchange Project, with Prof. P.Ormos, 2003-2006, 2000-2003, 1996-2000.
- Protein folding/unfolding machinery.
Unidad de Biofisica (CSIC-UPV/EHU) y Departamento de Bioquímica, Universidad del País Vasco, Bilbao, Spain, with Prof. A. Muga, 2000-2002.
- Spectral and structural changes in photosystem II after heat and light stress
Joint project with Sofia University, Faculty of Physics.
- Investigation of the interactions of some photosynthetic proteins with artificial lipid membranes, 2005-2007.
- Calorimetric investigations of poplar plastocyanins a and b (P. nigra). Establishment of methodology for isolation of photosystem I from the same plant species, 2005-2007.
- Peptide nanostructures as effectors and regulators of the oxidations and reduction processes in plant cells
Joint project of Sofia University and IBPhBME, BAS.
- Investigation of pathogenetic disturbances in lung carcinoma using proteomic analysis
Medical University-Sofia, 2008-2010
- Initial studies towards development of clinical biosensor for Alzheimer’s disease
NATO, № EAP.RIG.982718, 2007-2010
- Application of atomic force microscopy for the investigation of platelets derived from patients with deep venous thrombosis, carriers of trombocytic polymorphisms
2014 – 2016 joint project with Medical University – Pleven, Bulgaria
Project leader: Assoc. Prof. Regina Komsa_Penkova
- Thermodynamic behaviour of tumour cells. Effect of antitumour agents
Budgetary subsidy, 2014-2017
Coordinator: Prof. Stefka Taneva
- Morphology and topology of the main Light-harvesting complex of photosystem II (LHCII) studied by Brewster angle microscopy and Atomic force microscopy
Budgetary subsidy, 2014-2015
Coordinator: Sen. Assist Prof. Dr Tonia Andreeva
- Light-energy conversion and molecular dynamics in retinal proteins. Bioelectronic application
Bilateral cooperation – Hungary. Deadline, 2014-2015
Coordinator: Prof. Stefka Taneva
- Thermodynamic investigation of blood serum, novel approach for monitoring of haematological diseases (multiple myeloma)
Budgetary subsidy, 2011-2014
Coordinator: Prof. Stefka Taneva
- Unstructured proteins and Ewing Sarcoma
Budgetary subsidy, 2011-2014
Coordinator: Sen. Assist. Prof. Dr. Rumiana Todorova
- Evaluation of the effect of autologous stem cell transplantation on the status of patients with multiple myeloma by microcalorimetry
Program for Career Development of Young Scientists, Bulgarian Academy of Sciences, Project № ДФНП 140/2016
Coordinator: PhD student Avgustina Danailova
- Optimizing the biocompatibility of polyelectrolyte multilayer coatings made of hyaluronic acid and chitosan by introducing graphene oxide into the polymer matrix
Program for Career Development of Young Scientists, Bulgarian Academy of Sciences, Project ДФНП 139/2016
Coordinator: Res Fellow Svetozar Stoichev
- Optimized polyelectrolyte multilayer coatings for biofunctionalization of coronary stents
2014-2016 National Science Fund, № ДНТС 01/9
Project leader: Prof. Stefka G. Taneva
Collaborator: NMI Natural and Medical Sciences Institute at the University of Tübingen, Tübingen, Germany
International collaboration
- NMI Natural and Medical Sciences Institute at the University of Tübingen, Tübingen, Germany
- Laboratoire d’optique et biosciences, Ecole Polytechnique INSERM U696 – CNRS, Palaiseau, France
- Chimie et Biologie des Membranes et des Nanoobjets, CBMN-UMR, University of Bordeaux 1 “Science and Technology“, Bordeaux, France
- Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
- Unidad de Biofísica (CSIC/UPV) y Departamento de Bioquímica y Biología Molecular, Universidad del País Vasco, Bilbao, Spain
- Max Planck Institute of Colloids and Interfaces, Department of Interfaces, Research Campus Potsdam-Golm, Potsdam, Germany
Lecture courses
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- New developments and trends in protein science – Institute of Biophysics and Biomedical Engineering, BAS
Model membranes – Seminar series and laboratory exercises for the master students course, Faculty of Biology, Sofia University
Selected publications
- S. Todinova, S. Krumova, A. Danailova, V. Petkova, M.Guenova, G. Mihaylov, L. Gartcheva, S.G.Taneva, Calorimetric markers for monitoring of multiple myeloma and Waldenström’s macroglobulinemia patients, Eur. Biophys. J. 2018
https://doi.org/10.1007/s00249-018-1277-3
- N. Petrova, S.Todinova, H. Laczko-Dobos, T. Zakar, S. Vajravel, S. Taneva, Z.Gombos, S. Krumova, Structural integrity of Synechocystis sp. PCC 6803 phycobilisomes evaluated by means of differential scanning calorimetry, Photosynth. Res. 2018 https://rd.springer.com/article/10.1007/s11120-018-0481-4
- T.D. Andreeva, S. Stoichev, S.G. Taneva, R. Krastev, Hybrid graphene oxide/polysaccharide nanocomposites with controllable surface properties and biocompatibility, Carbohydrate Polymers 181, 2018, 78-85
- T.D. Andreeva, S.D. Petrova, K. Mladenova, V, Moskova-Doumanova, T. Topouzova-Hristova, Y. Petseva, N. Mladenov, K. Balashev, Z. Lalchev, J.A. Doumanov, Effects of Ca2+, Glu and GABA on hBest1 and composite hBest1/POPC surface films. Colloids and Surfaces B: Biointerfaces 161, 2018, 192-199
- L. Fábián, S. Krekic, R. Tóth-Boconádi, S.G. Taneva, A.M. Bálint, L. Nánai, and A. Dér Integrated optical investigation of two light-sensitive protein, TIM15-16 Physics Conference AIP Conf. Proc. 1796, 040001, 2017, doi: 10.1063/1.4972379, http://dx.doi.org/10.1063/1.4972379
- S. Krumova, S. Todinova, D. Mavrov, P. Marinov, V. Atanassova, K. Atanassov, S.G. Taneva, Intercriteria analysis of calorimetric data of blood serum proteome, Biochim. Biophys. Acta – General Subjects 1861, 2017, 409-417
- S. Todinova, R. Komsa-Penkova, S. Krumova, S.G. Taneva, G. Golemanov, G. Georgieva, P.Tonchev, B. Tsankov, L. Beshev, K. Balashev, T.D. Andreeva, PlA2 polymorphism in glycoprotein IIb/IIIa modulates the morphology and nanomechanics of platelets, Clinical and Applied Thrombosis/Hemostasis 1-10, 2017, 1076029616687847, https://doi.org/10.1177/1076029616687847
- S. Krumova, S. Todinova, D. Mavrov, P. Marinov, V. Atanassova, K. Atanassov, S.G. Taneva, Intercriteria analysis of calorimetric data of blood serum proteome,
Biochim. Biophys. Acta – General Subjects, 1861, 2017, 409-417
- K. Danova, V. Motyka, M. Todorova, A. Trendafilova, S. Krumova, P. Dobrev, T. Andreeva, T. Oreshkova, S. Taneva, L. Evstatieva, Effect of Cytokinin and Auxin Treatments on Morphogenesis, Terpenoid Biosynthesis, Photosystem Structural Organization, and Endogenous Isoprenoid Cytokinin Profile in Artemisia alba Turra In Vitro, Journal of Plant Growth Regulation, 2017, https://link.springer.com/article/10.1007/s00344-017-9738-y?wt_mc=Internal.Event.1.SEM.ArticleAuthorOnlineFirst
- K. Mladenova, S.D. Petrova, T.D. Andreeva, V. Moskova-Doumanova, T. Topouzova-Hristova, Y. Kalvachev, K. Balashev, S.S. Bhattacharya, C. Chakarova, Z. Lalchev, J.A. Doumanov, Effects of Ca2+ ions on bestrophin-1 surface films. Colloids and Surfaces B: Biointerfaces, 149, 2017, 226-232
- T.D. Andreeva, H. Hartmann, S.G. Taneva, R. Krastev, Regulation of the growth, morphology, mechanical properties and biocompatibility of natural polysaccharide-based multilayers by Hofmeister anions, J. Mater. Chem. B 4, 2016, 7043-7170
- S. Krumova, S. Todinova, M. Tileva, L. Bouzhir-Sima, M.H. Vos, U. Liebl, S.G. Taneva, Thermal stability and binding energetics of Thymidylate Synthase ThyX, International Journal of Biological Macromolecules 91, 2016, 560–567
- S. Todinova, S. Krumova, T. Andreeva, K. Dimitrova, L. Gartcheva, S.G. Taneva, Unusual thermal transition in the serum calorimetric profile of Bence Jones multiple myeloma: a case report, Cancer Research Froniters 2, 2016, 416-426, doi: 10.17980/2016.416
- S. Todinova, E. Stoyanova, S. Krumova, I. Iliev, S.G. Taneva, Calorimetric signatures of human cancer cells and their nuclei, Thermochimica Acta 625, 2016, 95-101
- T.D. Andreeva, S. Castano, S. Krumova, S. Lecomte, S.G. Taneva, Effect of Protonation on the Secondary Structure and Orientation of Plant Light-Harvesting Complex II Studied by PM-IRRAS, Langmuir 31, 2015, 11583-11590
- Arregi, J.A. Olazabal-Herrero, M. Alonso-Mariño, S.G. Taneva, J.A. Rodríguez, M.A. Urbaneja, S. Bañuelos, Leukemia-Associated Mutations in Nucleophosmin Alter Recognition by CRM1: Molecular Basis of Aberrant Transport, PLoS ONE10(6) (2015) e0130610
- S. Stoichev, S.B. Krumova, T. Andreeva, J.V. Busto, S. Todinova, K. Balashev, M. Busheva, F.M. Goñi, S.G. Taneva, Low pH Modulates the Macroorganization and Thermal Stability of PSII Supercomplexes in Grana Membranes, Biophysical Journal 108, 2015, 844–853
- S. Krumova, S. Todinova, A. Danailova, V. Petkova, K. Dimitrova, L. Gartcheva, S.G. Taneva, Calorimetric features of IgM gammopathies. Implication for patient’s diagnosis and monitoring, Thermochimica Acta 615, 2015, 23–29
- Andreeva, T.D., Krumova, S.B., Minkov, I.L., Busheva, M., Lalchev, Z., Taneva, S.G., Protonation-induced changes in the macroorganization of LHCII monolayers, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 2014, 196–203
- Krumova, S.B., Várkonyi, Zs., Lambrev, P.H., Kovács, L., Todinova, S.J., Busheva, M.C., Taneva, S.G., Garab, G., Heat- and light-induced detachment of the light-harvesting antenna complexes of photosystem I in isolated stroma thylakoid membranes, Journal of Photochemistry and Photobiology B: Biology, 137, 2014, 4–12
- Krumova S., M. Zhiponova, K. Dankov, V. Velikova, K. Balashev, T. Andreeva, E. Russinova, S. Taneva, Brassinosteroids regulate the thylakoid membrane architecture and the photosystem II function, Journal of Photochemistry and Photobiology B: Biology, 126, 2013, 97-104
- Krumova S., B. Rukova, S. Todinova, L. Gartcheva, V. Milanova, D. Toncheva, S.G. Taneva, Calorimetric monitoring of the serum proteome in schizophrenia patients, Thermochimica Acta, 572, 2013, 59-64.
- Bañuelos S., B. Lectez, S.G. Taneva, G. Ormaza, M. Alonso-Mariño, X. Calle, M.A. Urbaneja. Recognition of intermolecular G-quadruplexes by full length nucleophosmin. Effect of a leukaemia-associated mutation, FEBS Letters, 587(14), 2013, 2254-2259.
- Szalontai B., G. Nagy, S. Krumova, E. Fodor, T. Páli, S.G. Taneva, G. Garab, J. Peters, A. Dér. Hofmeister ions control protein dynamics, Biochimica et Biophysica Acta –General Subjects, 1830(10), 2013, 4564-4572.
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