Biomacromolecules and Biomolecular Interactions
Head of Department
Prof. Stefka Taneva, DSc.
- E-mail: sgtaneva
gmail.com
- Phone: (359 2) 9792625
- Fax: (359 2) 8723787
- Address: “Acad. G. Bonchev” Str., Block 21, Room 314
Sofia 1113, Bulgaria
Staff
- Prof. Mira Busheva, PhD, miraobzor.bio21.bas.bg
- Assoc. Prof. Sashka Krumova, PhD, sashkaobzor.bio21.bas.bg
- Assoc. Prof. Tonja Andreeva, PhD, t_andreevaabv.bg
- Senior Assist. Prof. Roumiana Todorova, PhD, todorova
bio21.bas.bg
- Senior Assist. Prof. Svetla Todinova, PhD , todinovaabv.bg
- Assist. Prof. Svetozar Stoichev, PhD, svetlio85abv.bg
- Lab. Assist. Iren Tzonova, MSc. , tz_irenabv.bg
- Lab. Assist. Ognjan Apostolov, MSc. , oapostolovgooglemail.com
- Assist. Prof. Nia Petrova, PhD student, petrova_niayahoo.com
- Avgustina Danailova, specialist, PhD student, avgustina_danailovaabv.bg
Main research topics
See detailed information >
Dynamics of biological macromolecules and structure/function relationship. Thermal stability of proteins and complex biological systems. Macroorganization of pigment-protein complexes, factors modulating their conformation, integrity and function (lipids, biogenic organic compounds, steroids, ion strength and others). Application of thermodynamics in medicine – microcalorimetry of serum proteome (screening technology for diagnostics and monitoring of wide range of diseases).
Research projects
Current projects
- Application of atomic force microscopy for the investigation of platelets derived from patients with deep venous thrombosis, carriers of trombocytic polymorphisms
2014 – 2016 joint project with Medical University – Pleven, Bulgaria
Project leader: Assoc. Prof. Regina Komsa_Penkova
- Optimized polyelectrolyte multilayer coatings for biofunctionalization of coronary stents
2014-2016 National Science Fund, № ДНТС 01/9
Project leader: Prof. Stefka G. Taneva
Collaborator: NMI Natural and Medical Sciences Institute at the University of Tübingen, Tübingen, Germany
- Thermodynamic behaviour of tumour cells. Effect of antitumour agents
Budgetary subsidy, 2014-2017
Coordinator: Prof. Stefka Taneva
- Morphology and topology of the main Light-harvesting complex of photosystem II (LHCII) studied by Brewster angle microscopy and Atomic force microscopy
Budgetary subsidy, 2014-2015
Coordinator: Sen. Assist Prof. Dr Tonia Andreeva
- Light-energy conversion and molecular dynamics in retinal proteins. Bioelectronic application
Bilateral cooperation – Hungary. Deadline, 2014-2015
Coordinator: Prof. Stefka Taneva
- Investigation of the stability of DNA metabolic enzymes and thier interactions with ligands by means of spectroscopic, biochemical and thermodynamic methods
Bulgarian Science Fund, ДРИЛА-01-11, 2013-2015
Coordinator: Prof. Mira Busheva
- Thermodynamic investigation of blood serum, novel approach for monitoring of haematological diseases (multiple myeloma)
Budgetary subsidy, 2011-2014
Coordinator: Prof. Stefka Taneva
- Unstructured proteins and Ewing Sarcoma
Budgetary subsidy, 2011-2014
Coordinator: Sen. Assist. Prof. Dr. Rumiana Todorova
Past projects
- Regulation of the functions and organization of the photosynthetic apparatus by brassinosteroids
Bulgarian National Science Fund, 2009-2013
- A detailed study of the role of yeast linker histone Hho1p in the processes of chromatin remodeling
Joint project with Institute of Molecular Biology, BAS
Bulgarian National Science Fund, 2009-2012
- Light-energy conversion and molecular dynamics in retinal proteins. Bioelectronical application
Academy Exchange Project, with Prof. P. Ormos, Institute of Biophysics, Hungarian Academy of Sciences, 2010-2015
- Optimized formation of monomolecular layers from the major light harvesting complex of photosystem II on the phase boundary buffer/air
Institute of Biophysics and Biomedical Engineering, BAS, 2011-2012
- Thermodynamic investigation of blood serum, novel approach for monitoring of haematological (multiple myeloma) diseases
Institute of Biophysics and Biomedical Engineering, BAS, 2011-2013
- Role of supramolecular organization of PSII – LHCII complex in isoprene emitting plants and its relation to thermoprotection
Joint project of IBPhBME and IPBG, BAS, 2010-2012
- Carotenoid-lipid interactions in liposomes and antennae complexes
Joint project of Sofia University and IBPhBME, BAS
- Phospholipase A2 and the lipid peroxidation in neurodegenerative diseases. How the model membranes can contribute to the clarification of the action mechanisms of lipid peroxidation on membrane organization
Bulgarian National Science Fund, 2010-2013
- Colloid aspects of nanoscience: nanostructures at interfaces in bulk and in three-phase contact zones
Bulgarian National Science Fund, Project module NT-01/03: „Modified liquid and solid surfaces with adjustable nanostructures and properties”, 2006-2010.
- Developing a biosensor using photoexcitable membranes
Indo-Bulgarian intergovernmental programme of cooperation in science and technolgy, F.M. University, Dept. of Biosciences and Biotechnology, with Prof. A. Misra, 2007-2009.
- Biofunctionalized surfaces at solid-liquid interfaces
Institute of Biophysics, Hungarian Academy of Sciences, Academy Exchange Project, with Prof. P.Ormos, 2006-2009.
- Molecular mechanism of biological light energy transduction
Institute of Biophysics, Hungarian Academy of Sciences, Academy Exchange Project, with Prof. P.Ormos, 2003-2006, 2000-2003, 1996-2000.
- Protein folding/unfolding machinery.
Unidad de Biofisica (CSIC-UPV/EHU) y Departamento de Bioquímica, Universidad del País Vasco, Bilbao, Spain, with Prof. A. Muga, 2000-2002.
- Spectral and structural changes in photosystem II after heat and light stress
Joint project with Sofia University, Faculty of Physics.
- Investigation of the interactions of some photosynthetic proteins with artificial lipid membranes, 2005-2007.
- Calorimetric investigations of poplar plastocyanins a and b (P. nigra). Establishment of methodology for isolation of photosystem I from the same plant species, 2005-2007.
- Peptide nanostructures as effectors and regulators of the oxidations and reduction processes in plant cells
Joint project of Sofia University and IBPhBME, BAS.
- Investigation of pathogenetic disturbances in lung carcinoma using proteomic analysis
Medical University-Sofia, 2008-2010
- Initial studies towards development of clinical biosensor for Alzheimer’s disease
NATO, № EAP.RIG.982718, 2007-2010
International collaboration
- NMI Natural and Medical Sciences Institute at the University of Tübingen, Tübingen, Germany
- Laboratoire d’optique et biosciences, Ecole Polytechnique INSERM U696 – CNRS, Palaiseau, France
- Chimie et Biologie des Membranes et des Nanoobjets, CBMN-UMR, University of Bordeaux 1 “Science and Technology“, Bordeaux, France
- Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary
- Unidad de Biofísica (CSIC/UPV) y Departamento de Bioquímica y Biología Molecular, Universidad del País Vasco, Bilbao, Spain
- Max Planck Institute of Colloids and Interfaces, Department of Interfaces, Research Campus Potsdam-Golm, Potsdam, Germany
Lecture courses
- New developments and trends in protein science – Institute of Biophysics and Biomedical Engineering, BAS
- Biomaterial science – Seminar series and laboratory exercises for the students course, Faculty of Chemistry, Sofia University
Selected publications
- Andreeva, T.D., Krumova, S.B., Minkov, I.L., Busheva, M., Lalchev, Z., Taneva, S.G., Protonation-induced changes in the macroorganization of LHCII monolayers, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 460, 2014, 196–203
- Krumova, S.B., Várkonyi, Zs., Lambrev, P.H., Kovács, L., Todinova, S.J., Busheva, M.C., Taneva, S.G., Garab, G., Heat- and light-induced detachment of the light-harvesting antenna complexes of photosystem I in isolated stroma thylakoid membranes, Journal of Photochemistry and Photobiology B: Biology, 137, 2014, 4–12
- Krumova S., M. Zhiponova, K. Dankov, V. Velikova, K. Balashev, T. Andreeva, E. Russinova, S. Taneva, Brassinosteroids regulate the thylakoid membrane architecture and the photosystem II function, Journal of Photochemistry and Photobiology B: Biology, 126, 2013, 97-104
- Krumova S., B. Rukova, S. Todinova, L. Gartcheva, V. Milanova, D. Toncheva, S.G. Taneva, Calorimetric monitoring of the serum proteome in schizophrenia patients, Thermochimica Acta, 572, 2013, 59-64.
- Bañuelos S., B. Lectez, S.G. Taneva, G. Ormaza, M. Alonso-Mariño, X. Calle, M.A. Urbaneja. Recognition of intermolecular G-quadruplexes by full length nucleophosmin. Effect of a leukaemia-associated mutation, FEBS Letters, 587(14), 2013, 2254-2259.
- Szalontai B., G. Nagy, S. Krumova, E. Fodor, T. Páli, S.G. Taneva, G. Garab, J. Peters, A. Dér. Hofmeister ions control protein dynamics, Biochimica et Biophysica Acta – General Subjects, 1830(10), 2013, 4564-4572.
- Todinova, S. Krumova, P. Kurtev, V. Dimitrov, L. Djongov, Z. Dudunkov, S.G. Taneva, Calorimetry-based profiling of blood plasma from colorectal cancer patients, Biochim. Biophys. Acta, 1820, 2012, 1879–1885.
- Taneva, S.G., S. Bañuelos, M.A. Urbaneja, 2012, Thermodynamic signatures of macromolecular complexes. Insights on the stability and interactions of nucleoplasmin, a nuclear chaperone. InTech Applications of Calorimetry in a Wide Context – Differential Scanning Calorimetry, Isothermal Titration Calorimetry and Microcalorimetry, ISBN 978-953-51-0947-1, Book edited by: Dr. Amal Ali Elkordy, University of Sunderland, UK
- Fernández-Higuero J.A., Acebrón S.P., Taneva S.G., del Castillo U., Moro F., Muga A., Allosteric communcation between the nucleotide binding domains of ClpB, Journal of Biological Chemistry, 286(29), 2011, 25547-25555
- Landeta O., Landajuela A., Gil D., Taneva S.G., DiPrimo C., Sot B., Valle M., Frolov V., Basañez G., Reconstitution of proapoptotic BAK function in liposomes reveals a dual role for mitochondrial lipids in the BAK-driven membrane permeabilization process, Journal of Biological Chemistry, 286, 2011, 8213-8230.
- S. Todinova, S. Krumova, L. Gartcheva, Ch. Robeerst, S. G. Taneva, Microcalorimetry of blood serum proteome: A modified interaction network in the multiple myeloma case, Anal. Chem., 2011, 83, 7992–7998.
- G. Nagy, J. Pieper, S. Krumova, L. Kovács, M. Trapp, G. Garab, J. Peters, Dynamic properties of photosystem II membranes at physiological temperatures characterized by elastic incoherent neutron scattering. Increased flexibility associated with the inactivation of the oxygen evolving complex, Photosynth. Res. (in press)
- S. B. Krumova, S. J. Todinova, A. G. Dobrikova, S. G. Taneva, Differential scanning calorimetry of photosynthetic membranes: Resolving contributions of the major photosynthetic complexes to the sequential thermal transitions, Trends Photochem. Photobiol., 2011, 12, 37-51.
- I. Zlatanov, A. Popova, Penetration of Lysozyme and Cytochrome C in Lipid Bilayer: Fluorescent Study, J. Membrane Biol., 2011, 242, 95–103.
- I. Arregi, J. Falces, S. Bañuelos, M. A. Urbaneja, S. G. Taneva, The Nuclear Transport Machinery Recognizes Nucleoplasmin-Histone Complexes, Biochemistry, 2011, 50, 7104-7110.
- I. T. Ivanov, A. Zheleva, I. Zlatanov, Anion exchanger and the resistance against thermal haemolysis, Int. J. Hyperthermia, 2011, 27(3), 286-96.
- I. Chakarska, S. Todinova, K. Idakieva, Investigation on chemical cross-linked collagen phosphoric acid hydrolysates with cyanuric chloride by differential scanning calorimetry, J. Thermal Anal. Cal., 2010, 102, 1-7.
- S. B. Krumova, S. P. Laptenok, J. W. Borst, Monitoring Photosynthesis in Individual Cells of Synechocystis sp. PCC 6803 on a Picosecond Timescale, Biophysical J., 2010, 99 (6), 2006-2015.
- S. B. Krumova, S. P. Laptenok, L. Kovacs, Digalactosyl-diacylglycerol-deficiency lowers the thermal stability of thylakoid membranes, Photosynthesis Research, 2010, 105 (3), 229-242.
- S. Taneva, F. Moro, A. Velazquez-Campoy, A. Muga, Energetics of nucleotide-induced DnaK conformational states, Biochemistry, 2010, 49, 1338–1345.
- R. Tóth-Boconádi, A. Dér, S. G. Taneva, L. Kesthelyi, Excitation of the M intermediates of wild-type bacteriorhodopsin and mutant D96N: temperature dependence of absorbance, electric responses and proton movements, Theoretical Chemistry Accounts, 2010, 125, 365-373.
- J. Falces, I. Arregi, P. V. Konarev, M. A. Urbaneja, D. I. Svergun, S. G. Taneva, S. Bañuelos, Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex, Biochemistry, 2010, 49, 9756-9769.
- M. I. Dimitrov, A. A. Donchev, A. Ch. Shosheva, V. I. Getov, N. P. Terezova, S. D. Stoichev, Isolation and Identification of Poplar Isoplastocyanins, Z. Naturforsch., 2010, 65c, 225–230.
- S. G. Taneva, S. Bañuelos, J. Falces, I. Arregi, A. Muga, P. V. Konarev, D. I. Svergun, A. Velázquez-Campoy, M. A. Urbaneja, A Mechanism for Histone Chaperoning Activity of Nuc leoplasmin: Thermodynamic and Structural Models, J. Mol. Biol., 2009, 393, 448-463.
- J. G. Petrov, T. D. Andreeva, H. Möhwald, Dipolar Interactions and Miscibility in Binary Langmuir Monolayers with Opposite Dipole Moments of the Hydrophilic Heads, Langmuir, 2009, 25 (6), 3659-3666.
- V. I. Getov, G. R. Toromanov, G. K. Kostov, M. I. Dimitrov, A. Ch. Shosheva, Reversible unfolding of poplar iso-plastocyanins, J. Therm. Anal. Calorim., 2009, 98, 877-883.
- T. D. Andreeva, J. G. Petrov, G. Brezesinski, H. Möhwald, Structure of the Langmuir Monolayers with Fluorinated Ethyl Amide and Ethyl Ester Polar Heads Creating Dipole Potentials of Opposite Sign, Langmuir, 2008, 24 (15), 8001-8007.
- S. B. Krumova, C. Dijkema, P. de Waard, Phase behaviour of phosphatidylglycerol in spinach thylakoid membranes as revealed by P-31-NMR, Biochimica et Biophysica Acta-Biomembranes, 2008, 1778 (4), 997-1003.
- S. B. Krumova, R. B. M. Koehorst, A. Bota, Temperature dependence of the lipid packing in thylakoid membranes studied by time- and spectrally resolved fluorescence of Merocyanine 540, Biochimica et Biophysica Acta-Biomembranes, 2008, 1778 (12), 2823-2833.
- A. Der, L. Kelemen, L. Fabian, S. G. Taneva, E. Fodor, T. Pali, A. Cupane, M. G. Cacace, J. J. Ramsden, Interfacial water structure controls protein conformation, J. Phys. Chem. B, 2007, 111, 5344-5350.
- J. G. Petrov, T. D. Andreeva, H. Möhwald, Fluorination of the Hydrophilic Head Accelerates the Collapse of the Monolayer but Stabilizes the Bilayer of a Long Chain Trifluoroethyl Ether on Water, Langmuir, 2006, 22, 4136-4143.
- K. Jung, A. Gannoun, B. Sitek, O. Apostolov, A. Schramm, H.E. Meyer, K. Stühler and W. Urfer, Statistical evaluation of methods for the analysis of dynamic protein expression data from a tumor study, RevStat-Stat. J., 2006, 4, 67-80.
- A. Shosheva, A. Donchev, M. Dimitrov, G. Kostov, G. Toromanov, V. Getov, E. Alexov, Comparative study of the stability of poplar plastocyanin isoforms, Biochem. Biophys. Acta, 2005, 1748 (1), 116-127.
- S. Krumova, S. Todinova, M. Busheva, S. G. Taneva, Kinetic nature of the thermal destabilization of LHCII macroaggregates, J. Photochem. Photobiol. B, 2005, 78, 165-170
- A. Schramm, J.H. Schulte, K. Astrahantseff, O. Apostolov, V. Limpt, H. Sieverts, S. Kuhfittig-Kulle, P. Pfeiffer, R. Versteeg, A. Eggert, Biological effects of TrkA and TrkB receptor signaling in neuroblastoma, Cancer Lett., 2005, 228(1-2),: 143-153.
- B. Sitek, O. Apostolov, K. Stuhler, K. Pfeiffer, H.E. Meyer, A. Eggert, A. Schramm, Identification of dynamic proteome changes upon ligand activation of Trk-receptors using two-dimensional fluorescence difference gel electrophoresis and mass spectrometry, Mol. Cell. Proteomics, 2005, 4(3), 291-299.
- A. Shosheva, A. Donchev, M. Dimitrov, I. Zlatanov, G. Toromanov, V. Getov, E. Alexov, Experimental and numerical study of the poplar plastocyanin isoforms using Tyr as a probe for electrostatic similarity and dissimilarity, Biochem. Biophys. Acta, 2004, 1698, 67-75.
- A. Schramm, O. Apostolov, B. Sitek, K. Pfeiffer, K. Stuhler, H.E. Meyer, W. Havers, A. Eggert, Proteomics: techniques and applications in cancer research, Klin. Padiatr., 2003, 215(6), 293-297.
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